bvs
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so after many nights sat in front of my screen researching i think i know enough to give it a go. ill start with test E and hopefully move on to EQ, Deca and Tren A. how does this sound?
Test E powder: 20g
GSO: 47ml
BA: 1.6ml
BB: 14.4ml
(Is a 18%BB 2% BA ratio good?)
Method:
Vial sterilisation: ill be using uncapped vials that i have left over from a previous experiment. Media container and beakers will use the same method
1. wash with lab detergent
2. rinse several times with water
3. final rinse with distilled water
4. bake in the oven at 500deg F for 2hrs
Septum sterilisation:
1. rinse in hydrogen peroxide
QUESTION: is that enough? any particular strength of peroxide?
OR should i rise with isopropyl alcohol for 10-15min then put in the oven at 180deg F until dry
Cooking method:
1. measure out 20g of powder using electric scales
2. add powder to beaker containing oil and solvents
3. stir
4. warm beaker in a pan of water until solution becomes clear
5. let solution cool
6. filter through .22um bottle top filter and into a glass media container
7. transfer from media container to vials
8. put in septa
9. cap and crimp
10. pressure cook at 15psi for 1-2hrs
Obviously my main concern is sterility, so have i missed anything? ill be wearing medical gloves and changing them when appropriate and will steralise the room as best as possible with alcohol and keep windows and doors closed. i am also familiar with aseptic technique from uni so ill be employing that at all times.
thanks for any help bros
Test E powder: 20g
GSO: 47ml
BA: 1.6ml
BB: 14.4ml
(Is a 18%BB 2% BA ratio good?)
Method:
Vial sterilisation: ill be using uncapped vials that i have left over from a previous experiment. Media container and beakers will use the same method
1. wash with lab detergent
2. rinse several times with water
3. final rinse with distilled water
4. bake in the oven at 500deg F for 2hrs
Septum sterilisation:
1. rinse in hydrogen peroxide
QUESTION: is that enough? any particular strength of peroxide?
OR should i rise with isopropyl alcohol for 10-15min then put in the oven at 180deg F until dry
Cooking method:
1. measure out 20g of powder using electric scales
2. add powder to beaker containing oil and solvents
3. stir
4. warm beaker in a pan of water until solution becomes clear
5. let solution cool
6. filter through .22um bottle top filter and into a glass media container
7. transfer from media container to vials
8. put in septa
9. cap and crimp
10. pressure cook at 15psi for 1-2hrs
Obviously my main concern is sterility, so have i missed anything? ill be wearing medical gloves and changing them when appropriate and will steralise the room as best as possible with alcohol and keep windows and doors closed. i am also familiar with aseptic technique from uni so ill be employing that at all times.
thanks for any help bros