Crisper Cas9 part 1 Introduction

[Thrawn]

I will post a multi-part series about Crisper. I would very much enjoy the thought provoking ideas and how we can push the envelope not only for our bodies but our mind.

What is CRISPR-Cas9?

CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.

What is CRISPR-Cas9?​

• CRISPR-Cas9 is a unique technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding or altering sections of the DNA? sequence.
• It is currently the simplest, most versatile and precise method of genetic manipulation and is therefore causing a buzz in the science world.

How does it work?​

• The CRISPR-Cas9 system consists of two key molecules that introduce a change (mutation?) into the DNA. These are:
  • an enzyme? called Cas9. This acts as a pair of ‘molecular scissors’ that can cut the two strands of DNA at a specific location in the genome so that bits of DNA can then be added or removed.
  • a piece of RNA? called guide RNA (gRNA). This consists of a small piece of pre-designed RNA sequence (about 20 bases long) located within a longer RNA scaffold. The scaffold part binds to DNA and the pre-designed sequence ‘guides’ Cas9 to the right part of the genome. This makes sure that the Cas9 enzyme cuts at the right point in the genome.
• The guide RNA is designed to find and bind to a specific sequence in the DNA. The guide RNA has RNA bases? that are complementary? to those of the target DNA sequence in the genome. This means that, at least in theory, the guide RNA will only bind to the target sequence and no other regions of the genome.
• The Cas9 follows the guide RNA to the same location in the DNA sequence and makes a cut across both strands of the DNA.
• At this stage the cell? recognises that the DNA is damaged and tries to repair it.
• Scientists can use the DNA repair machinery to introduce changes to one or more genes? in the genome of a cell of interest.

 

[dirtys1x]

This is a wonderful piece of biotechnology that is used everyday. I worked in research at the animal modeling core at my university where we generated an albumin specific Cre-recombinase rat model using CRISPR-Cas9.

Although this is a great technology. It’s not really relevant for us who have already been born. In its current state, creating the DNA template is great and all, and CRISPR allows us to perform gene knock-ins. However, we need to microinject the finished product into embryos. So really, we can’t do much to help US.

 

[RISE]

Crisper is such a weird name. Sounds like a dating app for burn victims.

 

[dirtys1x]

Crisper is such a weird name. Sounds like a dating app for burn victims.

Lol it actually stands for “Clustered regularly interspaced short palindromic repeats”

So I guess it gets the name by default

 

[bigdguy]

Although this is a great technology. It’s not really relevant for us who have already been born. In its current state, creating the DNA template is great and all, and CRISPR allows us to perform gene knock-ins. However, we need to microinject the finished product into embryos. So really, we can’t do much to help US.

Just read this. I remember I read a lot about this during a time period of my life. I was trying to figure out if we could "modify ourselves" or for "genetic doping." I saw a documentary where a man injected himself with plasmids that would inhibit myosin but then he said we would need to inject great amounts to actually get to change our DNA? Never had it clear since it is a pretty new technology and big words.

 

[dirtys1x]

Just read this. I remember I read a lot about this during a time period of my life. I was trying to figure out if we could "modify ourselves" or for "genetic doping." I saw a documentary where a man injected himself with plasmids that would inhibit myosin but then he said we would need to inject great amounts to actually get to change our DNA? Never had it clear since it is a pretty new technology and big words.

You certainly can.. although I think this is incredibly dangerous. Just injecting straight modified plasmids into your body is as about as accurate as trying to hit a target from 100s of meters away in the dark. You have no idea where the effects will take place and what exactly it’s going to do. When I was doing my research at the animal modeling core we were microinjecting the plasmids we created using a high powered microscope into tiny rat embryos. Even then, after the rats had grown, not even MOST of them showed expression of our model.

 

[j2048b]

I will post a multi-part series about Crisper. I would very much enjoy the thought provoking ideas and how we can push the envelope not only for our bodies but our mind.

What is CRISPR-Cas9?

CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.

What is CRISPR-Cas9?​

• CRISPR-Cas9 is a unique technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding or altering sections of the DNA? sequence.
• It is currently the simplest, most versatile and precise method of genetic manipulation and is therefore causing a buzz in the science world.

How does it work?​

• The CRISPR-Cas9 system consists of two key molecules that introduce a change (mutation?) into the DNA. These are:
  • an enzyme? called Cas9. This acts as a pair of ‘molecular scissors’ that can cut the two strands of DNA at a specific location in the genome so that bits of DNA can then be added or removed.
  • a piece of RNA? called guide RNA (gRNA). This consists of a small piece of pre-designed RNA sequence (about 20 bases long) located within a longer RNA scaffold. The scaffold part binds to DNA and the pre-designed sequence ‘guides’ Cas9 to the right part of the genome. This makes sure that the Cas9 enzyme cuts at the right point in the genome.
• The guide RNA is designed to find and bind to a specific sequence in the DNA. The guide RNA has RNA bases? that are complementary? to those of the target DNA sequence in the genome. This means that, at least in theory, the guide RNA will only bind to the target sequence and no other regions of the genome.
• The Cas9 follows the guide RNA to the same location in the DNA sequence and makes a cut across both strands of the DNA.
• At this stage the cell? recognises that the DNA is damaged and tries to repair it.
• Scientists can use the DNA repair machinery to introduce changes to one or more genes? in the genome of a cell of interest.

There is a china doctor getting out of prison for altering 3 embryos and remapping their dna so they did not get hiv from their dad....interested to see how this effects them as they grow....

Now since we all know most of those in power are replicas or clones, u can see them aging backwards or not at all


Look at tomb brady...dude hasnt aged except maybe a bit of grey hair

 

[bigdguy]

is as about as accurate as trying to hit a target from 100s of meters away in the dark.

It’s crazy to think about it like that. I am very curious how this technology is gonna develop in the future. I am very curious if there’s people/athletes crazy enough to experiment with themselves and if they have had results. They call themselves biohackers I think??

 

[dirtys1x]

It’s crazy to think about it like that. I am very curious how this technology is gonna develop in the future. I am very curious if there’s people/athletes crazy enough to experiment with themselves and if they have had results. They call themselves biohackers I think??

Oh there definitely are. I wouldn’t have enough balls for that.

 

[bigrobbie]

Crisper scares me shitless

 

[bigdguy]

Same. Just imagine the possibilities in the future. How are competition in sports gonna be everyone genetically modified? that could be either the end or the beginning…

 

[Rickrok]

Thanks for this interesting information!

 

[Rickrok]

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[BigBaldBeardGuy]

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[MisterSuperGod]

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Whoopsies. Is that why everything on my display is upside and backwards now?